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mightyscript plus first strand cdna synthesis master mix  (Sangon Biotech)

 
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    Sangon Biotech mightyscript plus first strand cdna synthesis master mix
    Mightyscript Plus First Strand Cdna Synthesis Master Mix, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mightyscript+first+strand+cdna+synthesis+master+mix/cdna+first+kit+mirna+strand+synthesis/pm42297343-57-21-34
    Average 86 stars, based on 1 article reviews
    mightyscript plus first strand cdna synthesis master mix - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    cDNA Synthesis:

    Article Title: A Multi-Omics Study Reveals the Active Components and Therapeutic Mechanism of Erhuang Quzhi Formula for Non-Alcoholic Fatty Liver Disease
    Article Snippet: Total RNA was extracted from mouse liver tissues using TRIzol reagent (B511311-0100, Sangon Biotech, Shanghai, China), and its concentration and purity were determined. .. According to the MightyScript First Strand cDNA Synthesis Master Mix (B639251-0100, Sangon Biotech), 1 μg of total RNA was reverse-transcribed into cDNA. .. Quantitative PCR was performed using SYBR Green master mix (RK21203, ABconal, Wuhan, China) on a real-time PCR system under the following conditions: 95 °C for 5 min; 40 cycles of 95 °C for 15 s and 60 °C for 30 s. The relative mRNA expression of target genes was normalized to Hprt and calculated using the 2 ^−ΔΔCt method.

    Article Title: Differential toll-like receptor 2 activation by Akkermansia muciniphila and Bacteroides thetaiotaomicron mediates the beneficial effects of Fu brick tea polysaccharide against colitis.
    Article Snippet: Total ribonucleic acid (RNA) was extracted from colon tissues and Caco-2 cells using TRIzol® Reagent (Thermo Fisher Scientific Inc., Cat# 15596026) and treated with DNase I (Thermo Fisher Scientific, Cat# EN0521) to remove genomic Deoxyribonucleic acid (DNA) contamination. .. Total RNA was reverse transcribed into cDNA using MightyScript First Strand cDNA Synthesis Master Mix (Sangon Biotech, Cat# B639251). .. PCR analysis was performed using TB Green® Premix Ex TaqTM II (Takara Bio Inc.,) on an ABI StepOnePlusTM Real-Time PCR System (Applied BiosystemsTM).

    Article Title: Arsenic metabolism in litchi chinensis and its effects on fruit quality.
    Article Snippet: Total RNA was extracted from samples using Polysaccharide and Polyphenol Plant RNA Extraction Kits (Servicebio, China) and quantified with a NanoDrop (Thermo Scientific). .. First-strand cDNA was synthesized from 1 μg of total RNA using the MightyScript First Strand cDNA Synthesis Master Mix (Sangon Biotech, China) following the provided protocol, then diluted tenfold with nuclease-free water. .. RT-qPCR was performed with TaqProbe qPCR-Multiplex Mastermix (Sangon) in a CFX Opus 96 real-time PCR system (Bio-Rad, USA) using primers shown in Table S2.

    Article Title: DNAH11 impairs memory via disrupted synaptic plasticity in noise-induced hidden hearing loss mice.
    Article Snippet: Total RNA was isolated from samples with the TSINGKE TSP413 RNAprep FastPure kit (Beijing Tsingke Biotechnology Co., Ltd.). .. According to the manufacturers' protocols, this RNA was then reverse-transcribed into cDNA using the MightyScript First Strand cDNA Synthesis Master Mix (Sangon Biotech, Shanghai, China). .. Quantitative real-time PCR assays were run on a LightCycler 480 system (Roche, Switzerland) with SYBR Green PCR Master Mix (Yeasen, Shanghai, China).

    Article Title: Simulated microgravity induces cerebral dysfunction by disturbing protective microbiota-metabolite-microglia signaling across the gut‒brain ax is
    Article Snippet: .. Subsequently, cDNA was synthesized by reverse transcribing 1 μg of total RNA using the MightyScript First Strand cDNA Synthesis Master Mix (Sangon Biotech, China), following the manufacturer’s protocols. .. Quantitative real-time PCR (qPCR) was performed on the LightCycler® 480 System (Roche, Switzerland) using the SGExcel FastSYBR qPCR Kit (Sangon Biotech, China), with reaction conditions optimized for target genes and internal controls.

    Article Title: Restoring Firmicutes-Associated Metabolites: A Gut-Brain Axis Approach to Alleviate Neuroinflammation and Oxidative Stress in Intracerebral Hemorrhage.
    Article Snippet: In 2021, stroke was identified as the third leading cause of mortality worldwide, surpassed only by ischemic heart disease and coronavirus disease 2019 (COVID-19).. However, according to the recently published Global Burden of Disease Study 2023 (GBD 2023), stroke has ascended to the second leading cause of death [1, 2].. Among the primary subtypes of stroke, intracerebral hemorrhage (ICH) presents with a lower incidence compared to ischemic stroke, yet it poses significantly higher risks for mortality and disability.

    Article Title: Fructooligosaccharide modulates the intestinal exosomal miRNAs to reshape the gut microbiota
    Article Snippet: Total RNA of the exosomes, cells and feces was extracted using TRIzol (CWBIO, China) as instructed, and the quality of the extracted RNA was assessed as A260/A280 ratio using NanoDrop One (Thermo Fisher Scientific, USA). .. The qualified RNA was reverse transcribed into complementary DNA using MightyScript First Strand cDNA Synthesis Master Mix (Sangon Biotech, Shanghai) and qPCRs were performed with the Cobas z480 analyzer (Roche, Switzerland) using 2×SG Fast qPCR Master Mix (Sangon Biotech, Shanghai). ..

    Article Title: DNAH11 impairs memory via disrupted synaptic plasticity in noise-induced hidden hearing loss mice
    Article Snippet: Total RNA was isolated from samples with the TSINGKE TSP413 RNAprep FastPure kit (Beijing Tsingke Biotechnology Co., Ltd.). .. According to the manufacturers' protocols, this RNA was then reverse-transcribed into cDNA using the MightyScript First Strand cDNA Synthesis Master Mix (Sangon Biotech, Shanghai, China). .. Quantitative real-time PCR assays were run on a LightCycler 480 system (Roche, Switzerland) with SYBR Green PCR Master Mix (Yeasen, Shanghai, China).

    Reverse Transcription:

    Article Title: A Multi-Omics Study Reveals the Active Components and Therapeutic Mechanism of Erhuang Quzhi Formula for Non-Alcoholic Fatty Liver Disease
    Article Snippet: Total RNA was extracted from mouse liver tissues using TRIzol reagent (B511311-0100, Sangon Biotech, Shanghai, China), and its concentration and purity were determined. .. According to the MightyScript First Strand cDNA Synthesis Master Mix (B639251-0100, Sangon Biotech), 1 μg of total RNA was reverse-transcribed into cDNA. .. Quantitative PCR was performed using SYBR Green master mix (RK21203, ABconal, Wuhan, China) on a real-time PCR system under the following conditions: 95 °C for 5 min; 40 cycles of 95 °C for 15 s and 60 °C for 30 s. The relative mRNA expression of target genes was normalized to Hprt and calculated using the 2 ^−ΔΔCt method.

    Article Title: Differential toll-like receptor 2 activation by Akkermansia muciniphila and Bacteroides thetaiotaomicron mediates the beneficial effects of Fu brick tea polysaccharide against colitis.
    Article Snippet: Total ribonucleic acid (RNA) was extracted from colon tissues and Caco-2 cells using TRIzol® Reagent (Thermo Fisher Scientific Inc., Cat# 15596026) and treated with DNase I (Thermo Fisher Scientific, Cat# EN0521) to remove genomic Deoxyribonucleic acid (DNA) contamination. .. Total RNA was reverse transcribed into cDNA using MightyScript First Strand cDNA Synthesis Master Mix (Sangon Biotech, Cat# B639251). .. PCR analysis was performed using TB Green® Premix Ex TaqTM II (Takara Bio Inc.,) on an ABI StepOnePlusTM Real-Time PCR System (Applied BiosystemsTM).

    Article Title: DNAH11 impairs memory via disrupted synaptic plasticity in noise-induced hidden hearing loss mice.
    Article Snippet: Total RNA was isolated from samples with the TSINGKE TSP413 RNAprep FastPure kit (Beijing Tsingke Biotechnology Co., Ltd.). .. According to the manufacturers' protocols, this RNA was then reverse-transcribed into cDNA using the MightyScript First Strand cDNA Synthesis Master Mix (Sangon Biotech, Shanghai, China). .. Quantitative real-time PCR assays were run on a LightCycler 480 system (Roche, Switzerland) with SYBR Green PCR Master Mix (Yeasen, Shanghai, China).

    Article Title: Restoring Firmicutes-Associated Metabolites: A Gut-Brain Axis Approach to Alleviate Neuroinflammation and Oxidative Stress in Intracerebral Hemorrhage.
    Article Snippet: In 2021, stroke was identified as the third leading cause of mortality worldwide, surpassed only by ischemic heart disease and coronavirus disease 2019 (COVID-19).. However, according to the recently published Global Burden of Disease Study 2023 (GBD 2023), stroke has ascended to the second leading cause of death [1, 2].. Among the primary subtypes of stroke, intracerebral hemorrhage (ICH) presents with a lower incidence compared to ischemic stroke, yet it poses significantly higher risks for mortality and disability.

    Article Title: Fructooligosaccharide modulates the intestinal exosomal miRNAs to reshape the gut microbiota
    Article Snippet: Total RNA of the exosomes, cells and feces was extracted using TRIzol (CWBIO, China) as instructed, and the quality of the extracted RNA was assessed as A260/A280 ratio using NanoDrop One (Thermo Fisher Scientific, USA). .. The qualified RNA was reverse transcribed into complementary DNA using MightyScript First Strand cDNA Synthesis Master Mix (Sangon Biotech, Shanghai) and qPCRs were performed with the Cobas z480 analyzer (Roche, Switzerland) using 2×SG Fast qPCR Master Mix (Sangon Biotech, Shanghai). ..

    Article Title: DNAH11 impairs memory via disrupted synaptic plasticity in noise-induced hidden hearing loss mice
    Article Snippet: Total RNA was isolated from samples with the TSINGKE TSP413 RNAprep FastPure kit (Beijing Tsingke Biotechnology Co., Ltd.). .. According to the manufacturers' protocols, this RNA was then reverse-transcribed into cDNA using the MightyScript First Strand cDNA Synthesis Master Mix (Sangon Biotech, Shanghai, China). .. Quantitative real-time PCR assays were run on a LightCycler 480 system (Roche, Switzerland) with SYBR Green PCR Master Mix (Yeasen, Shanghai, China).

    Synthesized:

    Article Title: Arsenic metabolism in litchi chinensis and its effects on fruit quality.
    Article Snippet: Total RNA was extracted from samples using Polysaccharide and Polyphenol Plant RNA Extraction Kits (Servicebio, China) and quantified with a NanoDrop (Thermo Scientific). .. First-strand cDNA was synthesized from 1 μg of total RNA using the MightyScript First Strand cDNA Synthesis Master Mix (Sangon Biotech, China) following the provided protocol, then diluted tenfold with nuclease-free water. .. RT-qPCR was performed with TaqProbe qPCR-Multiplex Mastermix (Sangon) in a CFX Opus 96 real-time PCR system (Bio-Rad, USA) using primers shown in Table S2.

    Article Title: Simulated microgravity induces cerebral dysfunction by disturbing protective microbiota-metabolite-microglia signaling across the gut‒brain ax is
    Article Snippet: .. Subsequently, cDNA was synthesized by reverse transcribing 1 μg of total RNA using the MightyScript First Strand cDNA Synthesis Master Mix (Sangon Biotech, China), following the manufacturer’s protocols. .. Quantitative real-time PCR (qPCR) was performed on the LightCycler® 480 System (Roche, Switzerland) using the SGExcel FastSYBR qPCR Kit (Sangon Biotech, China), with reaction conditions optimized for target genes and internal controls.

    Article Title: Restoring Firmicutes-Associated Metabolites: A Gut-Brain Axis Approach to Alleviate Neuroinflammation and Oxidative Stress in Intracerebral Hemorrhage.
    Article Snippet: In 2021, stroke was identified as the third leading cause of mortality worldwide, surpassed only by ischemic heart disease and coronavirus disease 2019 (COVID-19).. However, according to the recently published Global Burden of Disease Study 2023 (GBD 2023), stroke has ascended to the second leading cause of death [1, 2].. Among the primary subtypes of stroke, intracerebral hemorrhage (ICH) presents with a lower incidence compared to ischemic stroke, yet it poses significantly higher risks for mortality and disability.

    Real-time Polymerase Chain Reaction:

    Article Title: Fructooligosaccharide modulates the intestinal exosomal miRNAs to reshape the gut microbiota
    Article Snippet: Total RNA of the exosomes, cells and feces was extracted using TRIzol (CWBIO, China) as instructed, and the quality of the extracted RNA was assessed as A260/A280 ratio using NanoDrop One (Thermo Fisher Scientific, USA). .. The qualified RNA was reverse transcribed into complementary DNA using MightyScript First Strand cDNA Synthesis Master Mix (Sangon Biotech, Shanghai) and qPCRs were performed with the Cobas z480 analyzer (Roche, Switzerland) using 2×SG Fast qPCR Master Mix (Sangon Biotech, Shanghai). ..



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